The Different Calcium+2 Intensity Profile and Quality of Oocyte and Goat Sperms after Cryopreservation

International Ruminant Seminar: "Eco-friendly livestock production for sustainable agriculture"

农业科学;生态环境科学

Ciptadi, G.^1 ; Rahayu, S.^2 ; Fatchiyah, F.^2 ; Wahjuningsih, S.^1 ; Budiarto, A.^1 ; Nasich, M.^1 ; Putri, A.R.I.^2 ; Mudawamah, M.^3 ; Ihsan, M.N.^1

Faculty of Animal Husbandry, UB, Indonesia^1

Faculty of MIPA-Biology UB, Indonesia^2

Faculty of Animal Husbandry, UNISMA, Malang, Indonesia^3

关键词: Cryo-preservation;    Cryopreserved;    Freezing methods;    In-vitro;    Indonesians;    Intensity profiles;    Laser scanned;    Sperm quality;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/119/1/012036/pdf
DOI  :  10.1088/1755-1315/119/1/012036

来源: IOP

【 摘 要 】

This research aims were to study the effect of the oocyte and sperms cryopreservation of Indonesian local goat on the post-thawing quality and profile or characters of Calcium+2 intensity in relating with their fertility capacity. A study was conducted to test the freezing method and post-thawing viability both stock cells stored in the deep freezer and liquid nitrogen (-80°C of vs -196°C). A fertility test of sperms has been conducted through in vitro of sperm quality, while the oocytes cryopreserved test was done by in vitro maturation (IVM) rate (%). The profile of Calcium 2+ was performed and analysis by Confocal Laser Scanned Microscope (CLSM). The result showed that IVM rate of goat oocyte is considered lower when cryopreserved in -80°C than in -196°C. Meanwhile, sperm is considered having a good quality in 2 methods of cryopreservation with post-thawing motility > 40 % (SNI 2014). There is an important difference between Calcium intensity of fresh and post-thawing both for oocyte and spermatozoa. Calcium +2 profiles is varied individually on the peak of intensity, but it considered expressed the same profile of each fresh and post-thawing cell. In vitro fertilization test need to be performed to complete the viability and fertility competence of these sperm and oocyte freezing stocks.