Influence of Meiotic Stages on Developmental Competence of Goat' Oocyte After Vitrification

International Ruminant Seminar: "Eco-friendly livestock production for sustainable agriculture"

农业科学;生态环境科学

Wahjuningsih, S.^1 ; Ihsan, M.N.^1

Animal Husbandry Faculty, Brawijaya University, Indonesia^1

关键词: Cleavage rates;    Cryo-preservation;    Formation rates;    In-vitro;    Investigate effects;    Maturation stage;    Vitrification process;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/119/1/012029/pdf
DOI  :  10.1088/1755-1315/119/1/012029

来源: IOP

【 摘 要 】

This objective of this research was to investigate effect of goat oocyte meiotic stages on developmental competence after cryopreservation. Ovaries were collected from slaugterhouse and oocytes was aspirated from2-6 mm of follicles. Oocyte with compacted cumulus cells and evenly granulated ooplasm were selected for this experiment. The lenght of in vitro maturation before vitrification was 8 or 22 h in IVM media TCM 199 + FCS 10 % + PMSG 10 IU + hCG 10 IU at 38.5 °C in a humidified atmosphere of 5 % CO2in air and were vitrified. After vitrification process, GVBD and MII oocyte were matured for 18 or 4 h to fullfill 26 h maturation requirement and then oocytes were subjected to IVF and culture. Cleavage and blastocyst formation rate were to asses their developmental competence. Cleavage rates were obtained for both GVBD ( 56.78 %) and MII (69.64 % ) oocytes (P