FEBS Letters | |
Protease‐activated kinase II as the mediator of epidermal growth factor‐stimulated phosphorylation of ribosomal protein S6 | |
Perisic, Olga1  Traugh, Jolinda A.1  | |
[1] Department of Biochemistry, University of California, Riverside, CA 92521, USA | |
关键词: Protein kinase; Epidermal growth factor; Phosphorylation; Ribosome; Protease-activated kinase; Swiss 3T3 cell; EGF; epidermal growth factor; TPA; 12-O-tetradecanoyl phorbol-13-acetate; PAK II; protease activated kinase II; | |
DOI : 10.1016/0014-5793(85)80779-1 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Epidermal growth factor stimulates phosphorylation of ribosomal protein S6 in serum-starved Swiss 3T3 cells, leading to the formation of highly phosphorylated derivatives containing 4–5 phosphates. Two-dimensional analysis of tryptic phosphopeptides of S6 shows an identical pattern to the ones obtained previously in other cells in response to insulin and the tumor promoting phorbol ester, 12-O-tetradecanoyl phorbol-13-acetate. This suggests a common intracellular mediator of S6 phosphorylation by different growth promoting agents. It is proposed that the potential mediator of this phosphorylation is the Ca2+-independent, cAMP-independent protein kinase, protease activated kinase II, as shown by the extent of phosphorylation and the tryptic phosphopeptide maps of S6 with highly purified enzyme [(1983) J. Biol. Chem. 258, 13998-14002].
【 授权许可】
Unknown
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