Epidermal growth factor stimulates the phosphorylation of pyruvate kinase in fleshly isolated rat hepatocytes

FEBS Letters

McGivan, J.D.¹  Moule, S.K.¹ 

[1] Department of Biochemistry, School of Medical Sciences, University Walk, Bristol, BS8 1TD, UK

关键词: Pyruvate kinase;    Epidermal growth factor;    Cyclic AMP;    Phosphorylation;    Hepatocyte;    BSA;    bovine serum albumin;    CIPhScAMP;    8-(4-chlorophenylthio)adenosine 3′;    5′-monophosphate;    EDTA;    ethanediamine tetraacetic acid;    EGF;    epidermal growth factor;    MOPS;    N-morpholinopropane sulphonic acid;    SDS-PAGE;    sodium dodecyl sulphate polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(91)80198-C

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Epidermal growth factor (EGF) has previously been shown to stimulate gluconeogenesis in rat liver by decreasing the activity of pyruvate kinase [(1988) Biochem. J. 255, 361–364]. Here we investigate the mechanism underlying the inactivation of the enzyme. EGF was found to increase the incorporation of phosphate into pyruvate kinase, with maximal phosphorylation achieved only after 10 min in the presence of the growth factor. The increase in phosphorylation was not additive with the caused by cyclic AMP. Phosphoamino acid analysis of pyruvate kinase isolated from cells treated with EGF indicated that EGF phosphorylation solely on serine residues. The exact site of EGF-mediated phosphorylation has yet to be identified.

【 授权许可】

Unknown