Sarcolemmal phospholamban is phosphorylated in isolated rat hearts perfused with isoprenaline

FEBS Letters

Huggins, John P.¹  England, Paul J.¹ 

[1] Department of Biochemistry, University of Bristol Medical School, University Walk, Bristol BS8 1TD, England

关键词: Phospholamban;    Sarcolemma;    Calcium channel;    Cardiac muscle;    Phosphorylation;    Cyclic AMP;   

DOI  :  10.1016/0014-5793(83)80839-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Phosphorylation of phospholamban in cardiac sarcolemma is implicated in the increased influx of Ca²⁺ through the slow calcium channel induced by catecholamines. A method is described for the preparation of highly purified sarcolemmal vesicles from rat heart, and this has been used to examine the phosphorylation of phospholamban in ³²P_i-perfused rat hearts. Phospholamban phosphorylation is increased 3-fold after 30 s of perfusion with 0.1 μM isoprenaline. The time course of this increase precedes the inotropic response by 5–10 s.

【 授权许可】

Unknown