期刊论文详细信息
FEBS Letters | |
Sarcolemmal phospholamban is phosphorylated in isolated rat hearts perfused with isoprenaline | |
Huggins, John P.1  England, Paul J.1  | |
[1] Department of Biochemistry, University of Bristol Medical School, University Walk, Bristol BS8 1TD, England | |
关键词: Phospholamban; Sarcolemma; Calcium channel; Cardiac muscle; Phosphorylation; Cyclic AMP; | |
DOI : 10.1016/0014-5793(83)80839-4 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Phosphorylation of phospholamban in cardiac sarcolemma is implicated in the increased influx of Ca2+ through the slow calcium channel induced by catecholamines. A method is described for the preparation of highly purified sarcolemmal vesicles from rat heart, and this has been used to examine the phosphorylation of phospholamban in 32Pi-perfused rat hearts. Phospholamban phosphorylation is increased 3-fold after 30 s of perfusion with 0.1 μM isoprenaline. The time course of this increase precedes the inotropic response by 5–10 s.
【 授权许可】
Unknown
【 预 览 】
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