Early presence of phospholamban in developing chick heart

FEBS Letters

Vetter, Roland¹  Will-Shahab, Liane¹  Kemsies, Christel¹  Küttner, Irmgard¹  Will, Horst¹ 

[1] Central Institute of Heart and Circulation Research, GDR Academy of Sciences, Lindenberger Weg 70, DDR-1115 Berlin-Buch, GDR

关键词: Cardiac muscle;    Ontogenesis;    Sarcoplasmic reticulum;    Ca2+-transport;    Phospholamban;    Protein kinase;    SR;    sarcoplasmic reticulum;    cAMP-PrK;    cyclic AMP-dependent protein kinase;    C-subunit;    catalytic subunit of cyclic AMP-dependent protein kinase;    Ca-PrK;    Ca2+/calmodulin-dependent protein kinase;    DTT;    dithiothreitol;    PMSF;    phenylmethylsulfonylfluoride;    M r;    relative molecular mass;   

DOI  :  10.1016/0014-5793(82)80630-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Phosphorylation of phospholamban and development of reticular Ca²⁺ transport were studied in crude membrane preparations of embryonic, newborn and adult chick heart. Maximal phosphorylation of phospholamban by added catalytic subunit of cyclic AMP-dependent protein kinase increases from embryonic day 4–15. It decreases with further development. In the same membrane preparations active Ca²⁺-uptake into vesicles of sarcoplasmic reticulum rises from day 4–7 and decreases then slightly until day 20. A several-fold increase in Ca²⁺-transport activity occurs at the time of hatching. The data indicate separate genetic control for synthesis of phospholamban and sarcoplasmic reticulum Ca²⁺-ATPase.

【 授权许可】

Unknown