FEBS Letters | |
Reconstitution of the solubilized cardiac sarcoplasmic reticulum potassium channel Identification of a putative M r ∼80 kDa polypeptide constituent | |
Liu, Qi-Yi1  Shen, Win K.1  Strauss, Harold C.1  Lai, F.Anthony2  Meissner, Gerhard2  | |
[1] Departments of Medicine and Pharmacology, Duke University Medical Center, Durham, NC, USA;The Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC, USA | |
关键词: Potassium channel; Sarcoplasmic reticulum; Protein purification; Lipid bilayer; Cardiac muscle; Chaps; Chaps; 3-[(3cholamidopropyl)dimethylammonio]-1-propanesulfonate; | |
DOI : 10.1016/0014-5793(91)81092-M | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Recent evidence has indicated that potassium ion movement through sarcoplasmic reticulum (SR) K+ channels is an important countercurrent for Ca2+ release from SR. We used Chaps-solubilized SR vesicles and sucrose density gradient centrifugation to identify components of the canine cardiac SR K+ channel. To overcome the difficulty of the absence of a high-affinity specific ligand, we have successfully applied the planar lipid bilayer reconstitution technique to identify and functionally assay for the solubilized SR K+ channel. We found that Chaps solubilization of the channel did not change the protein's functional properties. The cardiac SR K+ channel sediments as a 15–20S protein complex. A polypeptide of M r ∼80 kDa was found to specifically comigrate with the 15–20S gradient fractions and might be a major constituent of the cardiac SR K+ channel.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912020295403ZK.pdf | 636KB | download |