Components required for membrane assembly of newly synthesized K+ channel KcsA

FEBS Letters

van Dalen, Annemieke²  Killian, J.Antoinette²  de Kruijff, Ben²  van der Laan, Martin¹  Driessen, Arnold J.M.¹ 

[1]Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands

[2]Department of Biochemistry of Membranes, Centre for Biomembranes and Lipid Enzymology, Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands

关键词: Assembly;    Lipid bilayer;    Membrane insertion;    Oligomerization;    Potassium channel;    Targeting;    IMV;    inner membrane vesicle;    SRP;    signal recognition particle;    TLE;    total Escherichia coli lipid extract;   

DOI  :  10.1016/S0014-5793(01)03278-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An Escherichia coli in vitro transcription–translation system was used to study the components involved in the biogenesis of the homotetrameric potassium channel KcsA. We show that a functional signal recognition particle pathway is essential for tetramer formation, probably to direct correct monomer insertion in the membrane. In the absence of YidC or at reduced SecYEG levels, KcsA assembly occurs with lower efficiency. Strikingly, the highest efficiency of tetramerization was observed when transcription–translation was carried out in the presence of pure lipid vesicles, demonstrating that a phospholipid bilayer is the minimal membrane requirement to form the KcsA tetramer. It is concluded that SecYEG and YidC are not required for the formation of tetrameric KcsA in vitro.

【 授权许可】

Unknown