Cytosolic factors mediate protein insertion into the peroxisomal membrane

FEBS Letters

Just, Wilhelm W.²  Heid, Hans¹  Diestelkötter, Petra²  Pause, Birgit² 

[1]Deutsches Krebsforschungszentrum, Abt. Zellbiologie, Heidelberg, Germany

[2]Biochemie-Zentrum, Universität Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany

关键词: Peroxisomal membrane protein;    Membrane insertion;    TRiC;   

DOI  :  10.1016/S0014-5793(97)00975-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Following in vitro translation of the 22 kDa peroxisomal membrane protein (Pmp22p), gel filtration analysis of the post-ribosomal supernatant revealed that Pmp22p forms two complexes. Complex I is of high molecular weight, results in a crosslinking product of 80 kDa, and by co-immunoprecipitation with anti-TCP1 antibody was identified as TRiC. In complex II Pmp22p was crosslinked to a yet unknown polypeptide of 40 kDa (P 40). This complex exhibited much higher efficiency to insert Pmp22p into the peroxisomal membrane compared to complex I. In a model we suggest that newly synthesized Pmp22p is first bound to TRiC before being transferred to P 40 which may function as a cytosolic Pmp22p receptor.

【 授权许可】

Unknown