Targeting of the 22 kDa integral peroxisomal membrane protein

FEBS Letters

Hunziker, Andreas²  Saffrich, Rainer³  Just, Wilhelm W.¹  Ansorge, Wilhelm³  Pause, Birgit¹ 

[1] Biochemie-Zentrum Heidelberg (BZH), Universität Heidelberg, Im Neuenheimer Feld 328, D-69120 Heidelberg, Germany;Abteilung Zellbiologie, Deutsches Krebsforschungszentrum, D-69120 Heidelberg, Germany;European Molecular Biology Laboratory Heidelberg, D-69117 Heidelberg, Germany

关键词: Peroxisome;    Peroxisomal membrane protein;    Pmp22p;    Peroxisomal membrane targeting;    Membrane targeting signal;   

DOI  :  10.1016/S0014-5793(00)01332-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Investigating targeting of the 22 kDa peroxisomal membrane protein (Pmp22p) to the peroxisomal membrane we have confined the targeting signal to amino acid residues 16–37 located in the N-terminal cytoplasmic tail. Comparison of Pmp22p orthologous sequences revealed a conserved motif Y3xL3xP3x(KQN) which might represent the core of this targeting signal not found so far in other Pmps. Fusion of the Pmp22p N-terminal tail to the C-terminal portion of Pmp22p which per se is not targeted to peroxisomes, conveys peroxisomal targeting. These data suggest that Pmp22p is targeted to peroxisomes by a new membrane targeting signal which is necessary and sufficient to target a polypeptide containing two transmembrane spans to peroxisomes.

【 授权许可】

Unknown