Brefeldin A interferes with peroxisomal protein sorting in the yeast Hansenula polymorpha

FEBS Letters

Harder, Wim¹  Kram, Anita M¹  Salomons, Florian A¹  Veenhuis, Marten¹  van der Klei, Ida J¹ 

[1] Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), Biological Center, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands

关键词: Brefeldin A;    Peroxisome;    Protein sorting;    Yeast;   

DOI  :  10.1016/S0014-5793(97)00679-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have studied the effect of brefeldin A (BFA), a fungal toxin that interferes with coated vesicle formation, on the biogenesis of peroxisomes in the yeast Hansenula polymorpha. Addition of BFA (20 μg/ml) to cultures of H. polymorpha partially inhibited the development of peroxisomes and resulted in the reversible accumulation of newly synthesized peroxisomal membrane and matrix proteins at the endoplasmic reticulum. In contrast, BFA did not interfere with the selective degradation of peroxisomes. Taken together, our data suggest that the ER plays a crucial role in peroxisome biogenesis in H. polymorpha, possibly in the biosynthesis of the peroxisomal membrane.

【 授权许可】

Unknown