The role of carbohydrates in vectorial exocytosis The secretion of the gp 80 glycoprotein complex in a ricin‐resistant mutant of MDCK cells

FEBS Letters

Koch-Brandt, Claudia¹  Parczyk, Karsten¹ 

[1] Institut für Biochemie, Abteilung Molekulare Genetik, Universität Frankfurt, Robert-Mayer-Straße 7–9, D-6000 Frankfurt/M, Germany

关键词: Epithelial cell;    Protein sorting;    Carbohydrate moiety;   

DOI  :  10.1016/0014-5793(91)80132-M

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In the polarized epithelial Madin-Darby canine kidney (MDCK) cell line an 80 kDa glycoprotein complex (gp 80) is sorted into the spical pathway or exocytosis and is secreted constitutively at the apical cell surface. The unglycosylated form or the protein complex is secreted in a nonpolar fashion at both surface domains [(1987) J. Cell. Biol. 105, 2735–2743]. Using ricin-resistant MDCK cells the role of the terminal galactose and slalic acid residues in the sorting of the gp 80 complex was analysed. The results suggest that the carbohydrate cores, rather than the ultimate or penultimate sugar residues, play a critical role in the, intracellular transport of this protein.

【 授权许可】

Unknown