Ion channels from the Bacillus subtilis plasma membrane incorporated into planar lipid bilayers

FEBS Letters

Carrasco, Alicia²  Wolff, Daniel¹  Venegas, Rodrigo¹  Alcayaga, Carmen¹ 

[1] Laboratorio de Fisiologia Celular, Departamento de Biologia, Universidad de Chile, Casilla 653, Santiago, Chile;Departamento de Ciencias Ecológicas, Faculdad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile

关键词: Ion channel;    Plasma membrane;    Lipid bilayer;    Bacteria;    Bacillus subtilis;   

DOI  :  10.1016/0014-5793(92)81112-Y

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Fusion of Bacillus subtilis plasma membrane vesicles with planar lipid bilayers induced the appearance of discrete current fluctuations characteristic of ion channels. These channels showed a wide range of conductances and kinetic behaviors. In 300 mM KCl, their conductances ranged from a few hundreds of pS to more than 1 nS, and most of them exhibited several sub-states. The channels poorly discriminated between small univalent anions and cations. Some of them showed voltage dependence and most of them presented a complex gating kinetics. The results are consistent with the hypothesis of the presence in the B. subtilis plasma membrane of pores composed of subunits that function cooperatively.

【 授权许可】

Unknown