The major Thiobacillus ferrooxidans outer membrane protein forms low conductance ion channels in planar lipid bilayers

FEBS Letters

Silva, Macarena¹  Rodriguez, Manuel¹  Wolff, Daniel²  Ferreira, Arianna¹ 

[1] Laboratorio de Microbiologia, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile;Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile

关键词: Ion channel;    Porin;    Lipid bilayer;    Autotrophic bacteria;    Thiobacillus ferrooxidans;   

DOI  :  10.1016/0014-5793(92)80372-N

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A protein isolated and purified from the outer membrane of the acidophilic, chemolithotrophic bacterium, Thiobacillus ferrooxidans with an oligomeric molecular weight of 90 000 Da (p9O) was incorporated into phosphatidylethanolamine planar lipid bilayers. The protein formed slightly anionic channels in KCl solutions, with a conductance of 25 pS in 100 mM KCl. The current—voltage relationship was linear between ±6O mV, and the conductance was a saturating function of the salt concentration. These channels fluctuated from a single open to closed state at low potentials, but present flickering activity at higher potentials.

【 授权许可】

Unknown