Purification and Characterization of Urease Obtained from Thiobacillus thiooxidans

4th International Conference on Applied Materials and Manufacturing Technology

材料科学;机械制造

Liu, Xiaojuan^1

Jiangsu Provincial Xuzhou Pharmaceutical Vocational College, Xuzhou, China^1

关键词: Autotrophic bacteria;    Optimum temperature;    Purified enzyme;    SDS PAGE;    Thiobacillus thiooxidans;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/423/1/012099/pdf
DOI  :  10.1088/1757-899X/423/1/012099

学科分类：材料科学（综合）

来源: IOP

【 摘 要 】

In this work, we described the separation of urease from Thiobacillus thiooxidans (T.t). Urease produced by T.t was purified to homogeneity by precipitation with 70% ethanol followed by column chromatography. The molecular weight of the purified enzyme was estimated to be 47,000 by SDS-PAGE. The enzyme exhibited a maximum activity at pH 5.6∼6.1. The optimum temperature was 37°C, with stability up to 40°C. Enzyme activity was improved strongly by Ni2+ and was reduced markedly in the presence of Co2+ and Pb2+. There is no relevant paper reported that found and purified urease from autotrophic bacteria in the world.