Enantioselectivity and Thermostability of a Novel Hyperhermotolerant Lipase from Geobacillus Thermodenitrificans nr68 (Lip.nr-68) on Secondary Racemic Alcohols Acetylation

3rd International Conference on Global Sustainability and Chemical Engineering

工业技术;化学工业

Nik Him, N.R.^1 ; Ibrahim, D.^2

Bioprocess Engineering Department, Faculty of Chemical Engineering, Universiti Teknologi MARA (UiTM), Selangor, Shah Alam

40450, Malaysia^1

Industrial Biotechnology Research Laboratory, School of Biological Sciences, Universiti Sains Malaysia, Pulau Pinang

11800, Malaysia^2

关键词: 1-Phenylethanol;    Geobacillus thermodenitrificans;    High activity;    Lipase enzyme;    Purified enzyme;    Racemic alcohols;    SDS-PAGE analysis;    Vinyl acetates;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/358/1/012031/pdf
DOI  :  10.1088/1757-899X/358/1/012031

学科分类：工业工程学

来源: IOP

【 摘 要 】

In our previous work, a new lipase enzyme has been purified from a species identified as a Gram negative Geobacillus thermodenitrificans nr68, isolated from a hot spring in Malaysia with growth temperature of 48°C. This new lipase, called Lip.nr-68 has been characterized as a hyperthermotolerant protein with high stability at 65°C and has been showing excellent characteristics that are very much comparable yet better than some of those of well-known industrially-used lipases. It shows high activity against long-chain triglycerides with molecular weight of the purified enzyme estimated to be 33.5 kDa using SDS-PAGE analysis. This paper is focusing on hyperthermotolerant Lip.nr-68 performance in promoting for enantioselectivity activities towards three secondary racemic alcohols namely 1-phenylethanol, 1-cyclohexilethanol and 1-(naft-2-il) ethanol by acetylation with vinyl acetate. Lip.nr-68 has been confirmed to show high and usual enantioselectivitiy according to the Kazlauskas Rule towards all secondary racemic alcohols and has significantly approved as an enantiomer selective biocatalyst towards 1-phenylethanol and 1-cyclohexylethanol at 65°C. Lip.nr-68 has showed a reduction of (R) and (S) enantiomers as well as the production of 68-98% ee and almost 94% yield of 3-4 mg/ml for 1-cyclohexilethanol.