Parameters for Novel Production of Fruity Floral Fragrance Ester (Geranyl Butyrate) by Locally Isolated Lipase Geobacillus thermodenitrificans nr68 (LGT)

3rd International Conference on Global Sustainability and Chemical Engineering

工业技术;化学工业

Nik Raikhan, N.H.^1

Faculty of Chemical Engineering, Universiti Teknologi MARA, Selangor, Shah Alam, Shah Alam

40450, Malaysia^1

关键词: Floral fragrances;    Fragrance industry;    Geobacillus thermodenitrificans;    Geranyl butyrates;    Linolenic acids;    Optimum parameters;    Substrate selection;    Thermotolerant lipase;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/358/1/012030/pdf
DOI  :  10.1088/1757-899X/358/1/012030

学科分类：工业工程学

来源: IOP

【 摘 要 】

Geranyl butyrate has been synthesized successfully using our locally isolated lipase Geobacillus thermodenitrificans nr68 (LGT) as the fragrance ester with aim to be used in a nanotechnology fragrance application. We have used and modified few parameters from the previous research and then, continued with optimization of the synthesis by looking into degree of esterification and water content in the system. Butyric acid (C4), stearic acid (C18: 0), caprylic acid (C8), linolenic acid (C18: 3), myristic acid (C14), linoleic acid (C18: 2) and oleic acid (C18: 1) were used in the substrate selection. The yield of geranyl butyrate before the optimization was 31.68±0.01%. The optimum parameters for the synthesis of geranyl butyrate were recorded as temperature of 65°C, shaking rate at 200 rpm, 5.0 ml of geraniol and 0.40 ml of butyric acid and 4.0 ml of n-butanol and 0.40 ml of oleic acid. After the optimization, geranyl butyrate synthesis was increased by 297% as to compare with the value before the parameters were optimized. We also have significantly reduced water content as a byproduct of the esterification and managed to run the system a success. The ability thermotolerant lipase from Geobacillus thermodenitrificans (LGT) in this synthesis is novel to Malaysian fragrance industry.