The first evidence of Acidithiobacillus albertensis in weathered ore samples from active gold mine Hodruša-Hámre (Slovakia)

Nova Biotechnologica et Chimica

Sedláková-Kaduková Jana¹  Pristaš Peter¹  Timková Ivana¹  Maliničová Lenka¹  Kisková Jana¹  Jeleň Stanislav² 

[1] Department of Microbiology, Institute of Biology and Ecology, Faculty of Science, Pavol Jozef Šafarik University in Košice, Šrobárova 2, SK-041 54 Košice, Slovak Republic;Earth Science Institute, Slovak Academy of Sciences, Ďumbierska 1, SK-974 01, Banská Bystrica, Slovak Republic;

关键词: Autotrophic bacteria;    Acidithiobacillus albertensis;    Gold mine;   

DOI  :  10.2478/nbec-2019-0003

来源: DOAJ

【 摘 要 】

Sulphur-oxidising autotrophic bacterial communities in deep biosphere from weathered ore samples from active gold mine Hodruša-Hámre, Slovakia were analysed using cultivation approach followed by DNA extraction, PCR amplification and 16S rRNA gene analyses. Indirect measurement of pH changes in cultivation media evidenced the presence of acidophilic bacteria with active production of acids. The decrease of pH was observed at the beginning of isolation and later pH in range of 1.5 – 2 was maintained in both, sulphuric acid and thiosulphate, media. The presence of homogenous population of gram-negative rods was proved by Gram staining. Molecular analyses have revealed that the population of sulphur-oxidising bacteria in gold mine is dominated by a single species of Aciditiobacillus genus, identified as A. albertensis, suggesting the low level of autotrophic bacteria diversity in deep deposits. For the first time this species was isolated from weathered rocks of a gold mine subsurface environment.

【 授权许可】

Unknown