Recombinant Protein Production from TPO Gen Cloning and Expression for Early Detection of Autoimmune Thyroid Diseases

International Conference on Chemistry and Material Science 2017

化学;材料科学

Aulanni'Am, Aulanni'Am^1,2 ; Wuragil, Dyah Kinasih^2 ; Soeatmadji, Djoko Wahono^3 ; Zulkarnain^3 ; Marhendra, Agung Pramana W.^1

Faculty of Sciences, Brawijaya University, Indonesia^1

Faculty of Veterinary Medicine, Brawijaya University, Indonesia^2

Faculty of Medicine, Brawijaya University Research Group of Biopeptide, Immunodetection and Vaccine (BIODET Vaccine), Brawijaya University, Indonesia^3

关键词: Cloning and expression;    Collaboration with industries;    Disease progression;    Immunochromatography;    Polyclonal antibody;    Protein purification;    Recombinant protein productions;    Thyroid disease;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/299/1/012013/pdf
DOI  :  10.1088/1757-899X/299/1/012013

学科分类：材料科学（综合）

来源: IOP

【 摘 要 】

Autoimmune Thyroid Disease (AITD) is an autoimmune disease that has many clinical symptoms but is difficult to detect at the onset of disease progression. Most thyroid autoimmune disease patients are positive with high titre of thyroid autoantibodies, especially thyroid peroxidase (TPO). The detection AITD are still needed because these tests are extremely high cost and have not regularly been performed in most of clinical laboratories. In the past, we have explored the autoimmune disease marker and it has been developed as source of polyclonal antibodies from patient origin. In the current study, we develop recombinant protein which resulted from cloning and expression of TPO gene from normal person and AITD patients. This work flows involves: DNA isolation and PCR to obtain TPO gene from human blood, insertion of TPO gene to plasmid and transformation to E. coli BL21, Bacterial culture to obtain protein product, protein purification and product analysis. This products can use for application to immunochromatography based test. This work could achieved with the goal of producing autoimmune markers with a guaranteed quality, sensitive, specific and economically. So with the collaboration with industries these devices could be used for early detection. Keywords: recombinant protein, TPO gene, Autoimmune thyroid diseases (AITD)ction of the diseases in the community.