Establishment of Colloidal Gold Immunochromatographic Assay for RHDV Antibody

7th Annual International Conference on Materials Science and Engineering

Zhou, Lijun^1 ; Luo, Yilin^1 ; Wang, Xiang^1 ; Song, Yong^2 ; Wu, Xulong^1 ; Zhang, Pengfei^1 ; Liao, Changyu^1 ; Jiang, Ruijiao^1 ; Yao, Xueping^1 ; Luo, Yan^1 ; Wang, Yin^1 ; Geng, Yi^1 ; Yang, Zexiao^2

Sichuan Agricultural University, Chengdu, China^1

Tianjin Ringpu Biotechnology Co. Ltd, Tianjin, China^2

关键词: False negative rate;    False positive rates;    Immunochromatography;    Polyclonal antibody;    Preparation conditions;    Rabbit hemorrhagic disease virus;    Rapid detection;    Reproducibilities;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/562/1/012129/pdf
DOI  :  10.1088/1757-899X/562/1/012129

来源: IOP

【 摘 要 】

This study aims to establish a rapid detection method for antibody against rabbit hemorrhagic disease virus (RHDV) by using immunochromatography technology. The colloidal gold-labeled recombinant VP60 protein, anti-VP60 polyclonal antibody and goat anti-rabbit IgG were coated on quality control line (C line) and detection line (T line). The test strip preparation conditions were optimized by detecting positive serum and negative serum. The results showed that the T-line and C-line were all red when tested positive serums. But only the C-line was red on the negative strips, the T-line was not colored, the false positive rate and the false negative rate were all zero percent. The specificity, sensitivity and reproducibility tests results all indicated that the colloidal gold strip is a powerful testing tool for the epidemiological investigation and detection of the antibody level on viral hemorrhagic disease in rabbits.