Experimental Design for a Macrofoam Swab Study Relating the Recovery Efficiency and False Negative Rate to Low Concentrations of Two Bacillus anthracis Surrogates on Four Surface Materials

Piepel, Gregory F. ; Hutchison, Janine R.

关键词: Bacillus anthracis;    Swab sampling;    False negative rate;    Recovery Efficiency;   

DOI  :  10.2172/1130248
RP-ID  :  PNNL-23094
PID  :  OSTI ID: 1130248
Others  :  Other: 400904120

美国|英语

来源: SciTech Connect

【 摘 要 】

This report describes the experimental design for a laboratory study to quantify the recovery efficiencies and false negative rates of a validated, macrofoam swab sampling method for low concentrations of Bacillus anthracis Sterne (BAS) and Bacillus atrophaeus (BG) spores on four surface materials (stainless steel, glass, vinyl tile, plastic light cover panel). Two analytical methods (plating/counting and polymerase chain reaction) will be used. Only one previous study has investigated false negative as a function of affecting test factors. The surrogates BAS and BG have not been tested together in the same study previously. Hence, this study will provide for completing gaps in the available information on the performance of macrofoam swab sampling at low concentrations.