Influence of hyperthermia on the phosphorylation of ribosomal protein S6 from human skin fibroblasts and meningioma cells

FEBS Letters

Issinger, O.-G.¹  Richter, W.W.¹  Zang, K.D.¹ 

[1] Institut für Humangenetik, Universität des Saarlandes, 6650 Homburg-3, FRG

关键词: Human;    Tumour cell;    Hyperthermia;    Phosphorylation;    Translation;    Ribosome;   

DOI  :  10.1016/0014-5793(83)80620-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Skin fibroblasts and meningioma cells, derived from primary cultures of the same patients have been used to study the influence of hyperthermia on (i) cell morphology and (ii) phosphorylation pattern of ribosomal and ribosome-associated proteins. Incubation of tumour cells and fibroblasts up to 7 h at 42°C did not significantly change the cell morphology as compared to control cells kept at 37°C. At 42°C ribosomal protein S6 is shifted cathodically indicating a loss of negative charge, however no quantitative dephosphorylation of S6 was observed. Meningioma cells and fibroblasts did not differ significantly with respect to S6 phosphorylation.

【 授权许可】

Unknown