cDNA cloning and chromosomal localization of the human α‐adrenergic receptor kinase

FEBS Letters

Benovic, J.L.¹  Croce, C.¹  Lefkowitz, R.J.²  Stone, W.C.²  Caron, M.G.¹  Huebner, K.¹ 

[1] Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, PA 19140, USA;Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA

关键词: β-Adrenergic receptor kinase;    Human;    Chromosome;    Desensitization;    Phosphorylation;    cDNA;   

DOI  :  10.1016/0014-5793(91)80568-N

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The β-adrenergic receptor kinase (βARK) mediates agonist-dependent phosphorylation of the β₂-adrenergic and related G protein-coupled receptors. A cDNA encoding bovine βARK has recently been isolated. In this work we have isolated a cDNA encoding human βARK from a retinal cDNA library. The cDNA encodes a protein of 689 amino acids with an overall 98.0% amino acid and 92.5% nucleotide identity with bovine βARK. Chromosomal location of the human βARK gene was determined by correlating the presence of the βARK locus with retention of a specific human chromosome in a rodent-human hybrid panel. This analysis revealed that the human βARK locus segregated with the long arm of chromosome 11, centromeric to 11q13.

【 授权许可】

Unknown