Dual regulation by G proteins of agonist‐dependent phosphorylation of muscarinic acetylcholine receptors

FEBS Letters

Haga, Kazuko¹  Haga, Tatsuya¹ 

[1]Department of Biochemistry, Institute of Brain Research, Faculty of Medicine, University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113, Japan

关键词: Muscarinic receptor;    Acetylcholine receptor;    GTP-binding regulatory protein;    Receptor phosphorylation;    β-Adrenergic receptor kinase;    Rhodopsin kinase;    G protein;    GTP-binding regulatory protein;    Go;    a G protein with α subunit of apparent molecular mass of 39 kDa;    [3H]QNB;    [3H]L-quinuclidinyl benzylate;    GTPγS;    guanosine 5 ' -(3-O-thio)triphosphate;    SDS-PAGE;    sodium dodecyl sulfate polyacrylamide gel electrophoresis;    Hepes;    4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid;    DTT;    dithiothreitol;   

DOI  :  10.1016/0014-5793(90)80968-O

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Muscarinic acetylcholine receptors purified from porcine atrium were phosphorylated, depending on the presence of agonists, by a protein kinase partially purified from porcine brain, which had similar properties to the β-adrenergic receptor kinase. GTP-binding regulatory proteins (Go) had dual effects on the phosphorylation of muscarinic receptors, i.e. stimulation at lower concentrations and inhibition at higher concentrations. The stimulatory effect was reproduced with the βγ subunit of Go and the inhibitory effect with the combination of the α and βγ subunits.

【 授权许可】

Unknown