Rapid agonist‐induced beta‐adrenergic receptor kinase translocation in C6 glioma cells

FEBS Letters

Garcia-Higuera, Irene¹  Mayor, Federico¹ 

[1] Departamento de Biologia Molecular, Centro de Biologia Molecular (CSIC-UAM), Universidad Autónoma, 28049 Madrid, Spain

关键词: β-Adrenergic receptor kinase;    Desensitization;    Translocation;    βAR(s);    β-adrenergic receptor(s);    βARK;    β-adrenergic receptor kinase;    PKA;    cAMP-dependent protein kinase;    SDS-PAGE;    sodium dodecyl sulfate polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(92)80285-O

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Exposure of C6 glioma cells to 1 μM isoproterenol leads to fast desensitization of the β-adrenergic receptor/adenylyl cyclase system and transient receptor sequestration. It also triggers a very rapid and transient translocation to the plasma membrane or β-adrenergic receptor kinase (βARK), a specific cytoplasmic kinase that phosphorylates only the agonist-occupied form of several G protein-coupled receptors. βARK-mediated receptor phosphorylation appears to be a suitable mechanism for the rapid regulation or adrenergic receptor function in the nervous tissue.

【 授权许可】

Unknown