The roles of intrinsic kinase and of kinase/activator protein in the enhanced phosphorylation of pyruvate dehydrogenase complex in starvation

FEBS Letters

Randle, Philip J.¹  Kerbey, Alan L.¹  Richardson, Lynne J.¹ 

[1] Department of Clinical Biochemistry, John Radcliffe Hospital, Oxford OX3 9DU, England

关键词: Pyruvate dehydrogenase complex;    Phosphorylation;    Kinase/activator;    Pyruvate dehydrogenase kinase;    Starvation;    Inactivation by phosphorylation;   

DOI  :  10.1016/0014-5793(84)80923-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Extracts of heart mitochondria from fed and from 48 h starved rats subjected to gel filtration on Sephacryl S-300 gave 4 major protein peaks. Pyruvate dehydrogenase complex eluted in the void volume and was assayed for intrinsic pyruvate dehydrogenase kinase activity which was increased approximately 3-fold by 48 h starvation of the rat. A second fraction, containing peaks 2 and 3 which overlapped, enhanced the activity of the intrinsic kinase and corresponds to kinase/activator protein described previously. Its activity was increased 1.5-fold by starvation.

【 授权许可】

Unknown