Long‐term regulation of pyruvate dehydrogenase kinase by high‐fat feeding

FEBS Letters

Orfali, Karen A.¹  Sugden, Mary C.¹  Holness, Mark J.¹  Fryer, Lee G.D.¹ 

[1] Department of Biochemistry, Faculty of Basic Medical Sciences, Queen Mary and Westfield College, University of London, Mile End Road, London, E1 4NS, UK

关键词: Pyruvate dehydrogenase complex;    Pyruvate dehydrogenase kinase;    Starvation;    High-fat diet;    Glucose/fatty acid cycle;    Insulin;    DBcAMP;    dibutyryl cyclic AMP;    NEFA;    non-esteri-fied fatty acids;    PDH;    pyruvate dehydrogenase;    PDHa;    the active form of PDH;   

DOI  :  10.1016/0014-5793(93)80864-Q

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The provision of the high-fat diet (47% of calories as fat) for 28 days evoked a significant decline in cardiac PDH_a, activity, together with marked increases in the activity of PDH kinase measured in isolated mitochondria and freshly-prepared cardiomyocytes from adult rats. Plasma insulin concentrations in fat-fed rats were not significantly different from control, but plasma NEFA concentrations were elevated. PDH kinase activity in cardiomyocytes from fat-fed rats fell substantially in culture (25 h). This decline was prevented by the inclusion of n-octanoate and DBcAMP in combination, but not individually, in the culture medium. The results are discussed in relation to the role for fatty acids and insulin in the long-term modulation of cardiac PDH kinase activity by high-fat feeding.

【 授权许可】

Unknown