Regulation of mammalian pyruvate dehydrogenase kinase

FEBS Letters

Popov, Kirill M¹ 

[1] Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, 635 Barnhill Drive, Indianapolis, IN 46202-5122, USA

关键词: Pyruvate dehydrogenase complex;    Pyruvate dehydrogenase kinase;    Regulation;    Metabolism of carbohydrates;    PDC;    pyruvate dehydrogenase complex;    PDK;    pyruvate dehydrogenase kinase;    RPDK2;    rat pyruvate dehydrogenase kinase;    isoenzyme 2;    E1;    pyruvate dehydrogenase component;    E2;    dihydrolipoyl acetyltransferase component;    E3;    dihydrolipoamide dehydrogenase;    E3BP;    E3-binding protein;   

DOI  :  10.1016/S0014-5793(97)01453-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

It is generally believed that mammalian pyruvate dehydrogenase kinase is a heterodimer consisting of catalytic and regulatory subunits. However, the contribution of the two subunits to the kinase-mediated signal transduction has remained undefined. In the present study recombinant components of mammalian pyruvate dehydrogenase complex were employed in order to characterize the role of the kinase catalytic subunit in the regulation of pyruvate dehydrogenase reaction. The results provide the first evidence strongly suggesting that the catalytic subunit of pyruvate dehydrogenase kinase is competent to respond to known effectors of kinase activity as well as to interact with the E₂-core without assistance of a regulatory subunit.

【 授权许可】

Unknown