FEBS Letters | |
Calmodulin binding and protein phosphorylation in adrenal medulla coated vesicles | |
Burgoyne, Robert D.1  Geisow, Michael J.1  | |
[1] National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, England | |
关键词: Ca2+ calmodulin; Chromaffin cell; Phosphorylation; Adrenal medulla; Clathrin; Coated vesicle; | |
DOI : 10.1016/0014-5793(84)80303-8 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Coated vesicles from bovine adrenal medulla contained clathrin and major detergent-insoluble polypeptides of 120-100, 51 and 49 kDa. Intact coated vesicles and vesicles lacking clathrin light chains were bound by immobilized calmodulin in the presence of Ca2+. Clathrin in the form of 700 Å cages was not bound. The calmodulin binding components in intact coated vesicles are therefore contributed by the enclosed vesicle or by the 120-100, 50 or 49 kda polypeptides. The 51 kDa component incorporated 32Pi from labelled ATP by an endogenous kinase activity; no other coat or vesicle membrane protein was phosphorylated in vitro, either by intrinsic or exogenous kinases.
【 授权许可】
Unknown
【 预 览 】
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