Presence of tropomyosin in adrenal chromaffin cells and its association with chromaffin granule membranes

FEBS Letters

Burgoyne, Robert D.¹  Norman, Kathyrn-Marie¹ 

[1] The Physiological Laboratory, University of Liverpool, PO Box 147, Liverpool L69 3BX, England

关键词: Chromaffin cell;    Tropomyosin;    Adrenal medulla;    Secretory granule;    Cytoskeleton;   

DOI  :  10.1016/0014-5793(85)80183-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The presence of tropomyosins in adrenal chromaffin cells was demonstrated by immunoperoxidase staining of cultured chromaffin cells by an anti-tropomyosin antibody. In immunoblotting the antibody labelled polypeptides of M _r 39000, 36500 and 31000 in 100000 × g supernatants and heat-stable fractions of adrenal medulla. The adrenal medullary tropomyosins did not comigrate with smooth muscle tropomyosins. The 39-kDa and 31-kDa polypeptides comigrated with polypeptides labelled by anti-tropomyosin in brain supernatants. Chromaffin granule membranes possessed a 39-kDa polypeptide that was labelled by anti-tropomyosin.

【 授权许可】

Unknown