Low molecular mass GTP‐binding proteins of adrenal chromaffin cells are present on the secretory granule

FEBS Letters

Burgoyne, Robert D.¹  Morgan, Alan¹ 

[1] Department of Physiology, University of Liverpool, PO Box 147, Liverpool L69 3BX, England

关键词: Secretory granule;    GTP-binding protein;    Chromaffin cell;    Secretion;    Exocytosis;   

DOI  :  10.1016/0014-5793(89)80204-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Adrenal medullary homogenates and chromaffin granule membranes were separated by SDS-polyacrylamide gel electrophoresis and GTP-binding proteins detected using [α-³²P]GTP binding to nitrocellulose blots. Four GTP-binding polypeptides of 24, 22, 20 and 18 kDa were routinely found in medullary homogenates and all were also found in isolated chromaffin granule membranes. The GTP-binding polypeptides co-sedimented with granule membrane markers following separation on sucrose gradients. On the basis of trypsin sensitivity and resistance to extraction, the GTP-binding proteins appeared to be tightly bound to the cytoplasmic surface of the granules. One or more of the secretory granule GTP-binding proteins could be involved in exocytosis in adrenal chromaffin cells.

【 授权许可】

Unknown