Nicotine‐evoked disassembly of cortical actin filaments in adrenal chromaffin cells

FEBS Letters

Burgoyne, Robert D.¹  Cheek, Timothy R.¹ 

[1] MRC Secretory Control Research Group, The Physiological Laboratory, University of Liverpool, PO Box 147, Liverpool L69 3BX, England

关键词: Chromaffin cell;    Actin filament;    Secretion;    Cytoskeleton;    Adrenal medulla;   

DOI  :  10.1016/0014-5793(86)80022-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using rhodamine-phalloidin staining it was found that actin filaments are concentrated in the cortex of resting chromaffin cells. Cortical actin filaments were disassembled 15 s after stimulation by nicotine and had reassembled 30 s later. Actin filament disassembly following nicotinic stimulation was also detected using the DNase I inhibition assay. Disassembly was independent of external calcium, insensitive to trifluoperazine and was not elicited by high K⁺, muscarinic agonists or phorbol ester. Disassembly of cortical actin filaments may allow access of secretory granules to exocytotic sites and act in conjunction with a rise in intracellular free calcium to bring about the full secretory response due to nicotinic agonists.

【 授权许可】

Unknown