Effects of mastoparan on catecholamine release from chromaffin cells

FEBS Letters

Wilson, Steven P.¹ 

[1]Department of Pharmacology, University of South Carolina School of Medicine, Columbia, SC 29208, USA

关键词: Adrenal medulla;    Chromaffin cell;    Mastoparan;    Secretion;    Catecholamine;    Peptide effect;   

DOI  :  10.1016/0014-5793(89)81343-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Release of catecholamines from bovine adrenal chromaffin cells exposed to mastoparan, a wasp venom peptide which activates GTP-binding proteins and phospholipase A₂, was evaluated. Release of catecholamines was dependent on mastoparan concentration and time of exposure. This release was, however, independent of extracellular calcium and accompanied by release of the cytoplasmic marker lactate dehydrogenase. Mastoparan also inhibited catecholamine secretion evoked by nicotine, but the peptide had little or no effect on release induced by other socretagogues. These findings suggest that in chromaffin cells mastoparan is not a secretagogue but rather causes cell lysis and blocks nicotinic receptor function.

【 授权许可】

Unknown