Effect of hexavalent carcinogenic chromium on carp Cyprinus carpio immune cells

Diseases of Aquatic Organisms

Jörn P. Scharsack¹  Tobias Helmus¹  Hans-Joachim Schuberth¹  R. Dinakaran Michael¹  Dieter Steinhagen¹  Wolfgang Leibold¹  Stephan Maurer¹  Andreas Skouras¹ 

关键词: Heavy metal;    Hexavalent chromium;    Cytotoxity;    Immunomodulation;   

DOI  :  10.3354/dao062155

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Chromium is widely used in industrial processes, and is released into aquatic environments by electroplating, tannery and textile industries. Fishes in natural waters or in aquaculture facilities supplied with these waters are exposed tochromium waste and are presumed to be affected by deposits. Herein, we examine the effect of hexavalent chromium on carp Cyprinus carpio derived immune cells. In vitro exposure of carp leukocytes to hexavalent chromium induced cytotoxicity,decreased mitogen-induced lymphocyte activation and phagocyte functions at concentrations between 2 and 200 µmol Cr l^–1. Neutrophils responded to chromium challenge by changes in cell shape together with reduced nitric oxide andreactive oxygen production. This occurred at much lower concentrations than for the cytotoxic effects seen in leukocyte cultures derived from peripheral blood or pronephros. In a similar way, activation of carp lymphocytes by pokeweed mitogen was reducedin a dose-dependent manner, while cytotoxic effects on non-activated lymphocytes were observed at much higher doses of 200 µmol Cr l^–l. Altered lymphocyte and neutrophil functions are considered to be responsible for decreasedresistance to pathogens observed in fishes under chronic chromium challenge.

【 授权许可】

Unknown