Study on the DNA-protein crosslinks induced by chromium (VI) in SPC-A1

2017 3rd International Conference on Environmental Science and Material Application

生态环境科学;材料科学

Liu, Yanqun^1 ; Ding, Jianjun^2 ; Lu, Xiongbing^1 ; You, Hao^1

School of Medicine, Jianghan University, Wuhan, China^1

School of Physics and Information Engineering, Jianghan University, Wuhan, China^2

关键词: Dna-protein crosslinks;    DNA-proteins;    Hexavalent chromium;    In-vitro;    Low concentrations;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/108/4/042096/pdf
DOI  :  10.1088/1755-1315/108/4/042096

来源: IOP

【 摘 要 】

Objective: This study was designed to investigate the effect of chromium (VI) on DNA-protein crosslinks (DPC) of SPC-A1 cells. Methods: We exposed SPC-A1 cells were cultured in 1640 medium and treated with the SPC-A1 cells in vitro to different concentrations of Hexavalent chromium Cr(VI) for 2h, the KC1-SDS precipitation assay were used to measure the DNA-protein cross-linking effect. Results: All the different concentrations of Cr(VI) could cause the increase of DPC coefficient in SPC-A1 cells. But this effect was not significant (P>0.05) at low concentrations; while in high concentration Cr(VI) induced SPC-A1 cells could produce DNA-protein cross-linking effect significantly (P