Pre-analytical stability of the plasma proteomes based on the storage temperature

Proteome Science

Luca Deiana⁵  Ciriaco Carru⁷  Giuseppe Castaldo⁴  Salvatore Sotgia⁷  Angelo Zinellu⁷  Giovannella Baggio¹  Claudio Franceschi³  Marta Deiana²  James Vaupel⁶  Sara Pinna⁷  Elisabetta Canu⁷  Angela Baralla⁷  Sara Pasella⁷ 

[1] Azienda Ospedaliera di Padova, Padua, Italy;Associazione “Isola dei Centenari”, Sassari, Italy;Dipartimento di Patologia Sperimentale, Università di Bologna, Bologna, Italy;Dipartimento di Biochimica e Biotecnologie Mediche, Universita’ di Napoli Federico II, Naples, Italy;Center for Biotechnology Development and Biodiversity Research, University of Sassari, Sassari, Italy;Max Planck Institute for Demographic Research, Rostock, Germany;Biochimica Clinica e Biologia Molecolare Clinica, Dipartimento di Scienze Biomediche, Università di Sassari, Sassari, Italy

关键词: Storage temperature;    Specimen collection and handling;    Plasma proteome;    Mass spectrometry;    Two-dimensional electrophoresis;   

Others  :  817058
DOI  :  10.1186/1477-5956-11-10

 received in 2012-10-04, accepted in 2013-03-18,  发布年份 2013

【 摘 要 】

Background

This study examined the effect of storage temperature on the protein profile of human plasma. Plasma samples were stored for 13 days at -80°C, -20°C, +4°C and room temperature (20-25°C) prior to proteomic analysis. The proteomic comparisons were based on the differences of mean intensity values of protein spots between fresh plasma samples (named “time zero”) and plasma samples stored at different temperatures. To better understand the thermally induced biochemical changes that may affect plasma proteins during storage we identified proteins with different expressions with respect to the time zero sample.

Results

Using two-dimensional electrophoresis followed by MALDI-TOF MS and /or LC-MS/MS 20 protein spots representing 10 proteins were identified with significant differences in abundance when stored at different temperatures. Our results, in agreement with various authors, indicate that during storage for a short period (13 days) at four different temperatures plasma proteins were more affected by degradation processes at +4°C compared to the other temperatures analysed. However, we founded that numerous protein spots (vitamin D binding protein, alpha-1-antitrypsin, serotransferrin, apoplipoprotein A-I, apolipoprotein E, haptoglobin and complement factor B) decrease in abundance with increasing temperature up to 4°C, but at room temperature their intensity mean values are similar to those of time zero and -80°C. We hypothesize that these proteins are labile at 4°C, but at the same time they are stable at room temperature (20-25°C). Furthermore we have grouped the proteins based on their different sensitivity to the storage temperature. Spots of serum albumin, fibrinogen gamma chain and haptoglobin are more resistant to the higher temperatures tested, as they have undergone changes in abundance only at room temperature; conversely, other spots of serum albumin, fibrinogen beta chain and serotransferrin are more labile as they have undergone changes in abundance at all temperatures except at -80°C.

Conclusions

Although there are many studies concerning protein stability of clinical samples during storage these findings may help to provide a better understanding of the changes of proteins induced by storage temperature.

【 授权许可】

   
2013 Pasella et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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