Utilization of mass spectrometric techniques to identify novel lipid biomarkers of traumatic brain injury and other practical applications

Traumatic brain injury;Biomarkers;Lipids;Mass spectrometry;Lipidomics

Hogan, Scott R. ; Fernández, Facundo M. LaPlaca, Michelle Chemistry and Biochemistry Finn, M. G. Merrill, Alfred Garg, Neha ; Fernández, Facundo M.

University:Georgia Institute of Technology

Department:Chemistry and Biochemistry

关键词: Traumatic brain injury;    Biomarkers;    Lipids;    Mass spectrometry;    Lipidomics;   

Others  :  https://smartech.gatech.edu/bitstream/1853/62721/1/HOGAN-DISSERTATION-2020.pdf

美国|英语

来源: SMARTech Repository

【 摘 要 】

The primary focus of this thesis centers around the examination of lipids to aid in the diagnosis, prognosis, and mechanistic understanding of traumatic brain injury (TBI). Lipids are important signaling molecules and play a critical role in energy storage as well as membrane structure and organization. Using mass spectrometry (MS) based methods to perform non-targeted metabolomic experiments with a focus on extraction of lipid metabolites, a rodent model is investigated to explore the feasibility of developing lipid biomarker panels to classify injury post-hoc across multiple severities. The developed methods are then used to study another biological system in order to show the broad applicability of lipidomics by investigating the effect of Karenia brevis allelopathy on two phytoplankton competitors.