A bio-synthetic interface for discovery of viral entry mechanisms.

Gutzler, Mike ; Maar, Dianna ; Negrete, Oscar ; Hayden, Carl C. ; Sasaki, Darryl Yoshio ; Stachowiak, Jeanne C. ; Wang, Julia

Sandia National Laboratories

关键词: Viruses;    Interfaces;    59 Basic Biological Sciences;    Membrane Transport;    Lipids;   

DOI  :  10.2172/1008149
RP-ID  :  SAND2010-6472
RP-ID  :  AC04-94AL85000
RP-ID  :  1008149

美国|英语

来源: UNT Digital Library

【 摘 要 】

Understanding and defending against pathogenic viruses is an important public health and biodefense challenge. The focus of our LDRD project has been to uncover the mechanisms enveloped viruses use to identify and invade host cells. We have constructed interfaces between viral particles and synthetic lipid bilayers. This approach provides a minimal setting for investigating the initial events of host-virus interaction - (i) recognition of, and (ii) entry into the host via membrane fusion. This understanding could enable rational design of therapeutics that block viral entry as well as future construction of synthetic, non-proliferating sensors that detect live virus in the environment. We have observed fusion between synthetic lipid vesicles and Vesicular Stomatitis virus particles, and we have observed interactions between Nipah virus-like particles and supported lipid bilayers and giant unilamellar vesicles.