Downregulation of Fetuin-B and Zinc-α2-glycoprotein is Linked to Impaired Fatty Acid Metabolism in Liver Cells

Cellular Physiology and Biochemistry

关键词: Plasma proteome;    High fat diet;    Obesity-prone and -resistant rats;    Fetuin-B;    Zinc-α2-glycoprotein;    RNA interference;   

DOI  :  10.1159/000339065

学科分类：分子生物学，细胞生物学和基因

来源: S Karger AG

【 摘 要 】

Background Our recent proteomic study has shown that plasma protein levels of fetuin-B (Ft-B) and zinc-α2-glycoprotein (ZAG) are significantly elevated in obesity-resistant (OR) rats exposed to a high fat diet. Time profiling of the plasma concentrations of Ft-B and ZAG in OR rats has shown stable regulation of these proteins throughout the entire period of rat breeding. Methods To firmly establish roles for these proteins in lipogenesis, we efficiently knocked down (KD) the genes FETUB and AZGP1 encoding Ft-B and ZAG, respectively, using siRNA in Chang liver cells. Results Reduced expression of FETUB and AZGP1 led to a significant increase in the expression of lipogenic genes, thereby resulting in higher lipid levels in both KD cells. Collectively with our previous findings, we confirmed that Ft-B was similarly regulated with Ft-A, in that their plasma protein levels were commonly reduced in diet-induced obese rats. Conclusion Our results provide a possible relationship between reduced plasma protein levels of Ft-B and ZAG and higher risk of diet-induced obesity through impaired fatty acid metabolism in hepatocytes.

【 授权许可】

CC BY-NC-ND