Detection and molecular typing of Leishmania tropica from Phlebotomus sergenti and lesions of cutaneous leishmaniasis in an emerging focus of Morocco

Particle and Fibre Toxicology

Meryem Lemrani¹  Myriam Riyad²  Abderahmane Laamrani El-Idrissi⁴  Salsabil Hamdi¹  Nargys Es-sette¹  Malika Ajaoud³ 

[1] Laboratoire de Parasitologie et Maladies Vectorielles, Institut Pasteur du Maroc, 1 Place Louis Pasteur, 20360 Casablanca, Morocco;Laboratoire de Parasitologie-Mycologie, Faculté de Médecine et de Pharmacie, Université Hassan II, Casablanca, Morocco;CEDoc des Sciences de la Santé, Faculté de Médecine et de Pharmacie, Université Hassan II, Casablanca, Morocco;Service des Maladies Parasitaires, Direction de l’Epidémiologie et de la Lutte contre les Maladies, Ministère de la Santé, Rabat, Morocco

关键词: Morocco;    Leishmania tropica;    Genotyping;    Phlebotomus sergenti;    Cutaneous leishmaniasis;   

Others  :  1225304
DOI  :  10.1186/1756-3305-6-217

 received in 2013-04-12, accepted in 2013-07-19,  发布年份 2013

【 摘 要 】

Background

Cutaneous leishmaniasis is an infectious disease caused by flagellate protozoa of the genus Leishmania. In Morocco, anthroponotic cutaneous leishmaniasis due to Leishmania tropica is considered as a public health problem, but its epidemiology has not been fully elucidated. The main objective of this study was to detect Leishmania infection in the vector, Phlebotomus sergenti and in human skin samples, in the El Hanchane locality, an emerging focus of cutaneous leishmaniasis in central Morocco.

Methods

A total of 643 sand flies were collected using CDC miniature light traps and identified morphologically. Leishmania species were characterized by ITS1 PCR-RFLP and ITS1-5.8S rRNA gene nested-PCR of samples from 123 females of Phlebotomus sergenti and 7 cutaneous leishmaniasis patients.

Results

The sand flies collected consisted of 9 species, 7 of which belonged to the genus Phlebotomus and two to the genus Sergentomyia. Phlebotomus sergenti was the most predominant (76.67%).

By ITS1 PCR-RFLP Leishmania tropica was found in three Phlebotomus sergenti females and four patients (4/7). Using nested PCR Leishmania tropica was identified in the same three Phlebotomus sergenti females and all the 7 patients. The sequencing of the nested PCR products recognized 7 haplotypes, of which 6 have never been described.

Conclusions

This is the first molecular detection and identification of Leishmania tropica in human skin samples and Phlebotomus sergenti in support of its vector status in El Hanchane. The finding of seven Leishmania tropica haplotypes underscores heterogeneity of this species at a high level in Morocco.

【 授权许可】

   
2013 Ajaoud et al.; licensee BioMed Central Ltd.

【 图 表 】

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