Insecticide susceptibility status of Phlebotomus (Paraphlebotomus) sergenti and Phlebotomus (Phlebotomus) papatasi in endemic foci of cutaneous leishmaniasis in Morocco

Particle and Fibre Toxicology

Btissam Ameur¹  Mohammed El Rhazi²  Lhousseine Lakraa²  Mohammed El Elkohli²  El Bachir Adlaoui²  Souad Ouahabi²  Chafika Faraj² 

[1] Service de lutte Antivectorielle, Direction de l'Epidémiologie et de Lutte contre les Maladies, Agdal, Rabat 10080, Morocco;Laboratoire d'Entomologie Médicale, Institut National d'Hygiène, 27 Avenue Ibn Batouta, Agdal, Rabat 10090, Morocco

关键词: Morocco;    Phlebotomus papatasi;    Phlebotomus sergenti;    Malathion;    DDT;    Lambdacyhalothrin;    Insecticide susceptibility;    Sandflies;   

Others  :  1233049
DOI  :  10.1186/1756-3305-5-51

 received in 2011-12-01, accepted in 2012-03-19,  发布年份 2012

【 摘 要 】

Background

In Morocco, cutaneous leishmaniasis is transmitted by Phlebotomus sergenti and Ph. papatasi. Vector control is mainly based on environmental management but indoor residual spraying with synthetic pyrethroids is applied in many foci of Leishmania tropica. However, the levels and distribution of sandfly susceptibility to insecticides currently used has not been studied yet. Hence, this study was undertaken to establish the susceptibility status of Ph. sergenti and Ph. papatasi to lambdacyhalothrin, DDT and malathion.

Methods

The insecticide susceptibility status of Ph. sergenti and Ph. papatasi was assessed during 2011, following the standard WHO technique based on discriminating dosage. A series of twenty-five susceptibility tests were carried out on wild populations of Ph. sergenti and Ph. papatasi collected by CDC light traps from seven villages in six different provinces. Knockdown rates (KDT) were noted at 5 min intervals during the exposure to DDT and to lambdacyhalothrin. After one hour of exposure, sandflies were transferred to the observation tubes for 24 hours. After this period, mortality rate was calculated. Data were analyzed by Probit analysis program to determine the knockdown time 50% and 90% (KDT50 and KDT90) values.

Results

Study results showed that Ph.sergenti and Ph. papatasi were susceptible to all insecticides tested. Comparison of KDT values showed a clear difference between the insecticide knockdown effect in studied villages. This effect was lower in areas subject to high selective public health insecticide pressure in the framework of malaria or leishmaniasis control.

Conclusion

Phlebotomus sergenti and Ph. papatasi are susceptible to the insecticides tested in the seven studied villages but they showed a low knockdown effect in Azilal, Chichaoua and Settat. Therefore, a study of insecticide susceptibility of these vectors in other foci of leishmaniasis is recommended and the level of their susceptibility should be regularly monitored.

【 授权许可】

   
2012 Faraj et al; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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