IL-12 based gene therapy in veterinary medicine

Journal of Translational Medicine

Natasa Tozon¹  Gregor Sersa²  Maja Cemazar²  Darja Pavlin¹ 

[1] University of Ljubljana, Veterinary Faculty, Small Animal Clinic, Cesta v Mestni log 47, Ljubljana, 1000, Slovenia;Institute of Oncology Ljubljana, Department for Experimental Oncology, Zaloska 2, Ljubljana, 1000, Slovenia

关键词: Electroporation;    Plasmid;    Horses;    Cats;    Dogs;    Gene therapy;    Interferon-γ;    Interleukin-12;   

Others  :  1205892
DOI  :  10.1186/1479-5876-10-234

 received in 2012-08-03, accepted in 2012-10-18,  发布年份 2012

【 摘 要 】

The use of large animals as an experimental model for novel treatment techniques has many advantages over the use of laboratory animals, so veterinary medicine is becoming an increasingly important translational bridge between preclinical studies and human medicine. The results of preclinical studies show that gene therapy with therapeutic gene encoding interleukin-12 (IL-12) displays pronounced antitumor effects in various tumor models. A number of different studies employing this therapeutic plasmid, delivered by either viral or non-viral methods, have also been undertaken in veterinary oncology. In cats, adenoviral delivery into soft tissue sarcomas has been employed. In horses, naked plasmid DNA has been delivered by direct intratumoral injection into nodules of metastatic melanoma. In dogs, various types of tumors have been treated with either local or systemic IL-12 electrogene therapy. The results of these studies show that IL-12 based gene therapy elicits a good antitumor effect on spontaneously occurring tumors in large animals, while being safe and well tolerated by the animals. Hopefully, such results will lead to further investigation of this therapy in veterinary medicine and successful translation into human clinical trials.

【 授权许可】

   
2012 Pavlin et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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