In vivo transfection of testicular germ cells and transgenesis by using the mitochondrially localized jellyfish fluorescent protein gene

FEBS Letters

Nakatsuji, Norio¹  Sakurai, Takayuki¹  Chuma, Shinichiro¹  Saito, Tetsuichiro¹  Huang, Zhenyong¹  Tamura, Masaru¹ 

[1] Mammalian Development Laboratory, National Institute of Genetics, Mishima 411-8540, Japan

关键词: Transgenesis;    Testis;    Spermatozoon;    Green fluorescent protein/yellow fluorescent protein;    Electroporation;    Intracytoplasmic injection;    ICSI;    intracytoplasmic sperm injection;    GFP;    green fluorescent protein;    YFP;    yellow fluorescent protein;   

DOI  :  10.1016/S0014-5793(00)02271-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We aimed to introduce foreign DNA into spermatogenic cells in the testis by injection of the DNA encoding jellyfish fluorescent proteins, green fluorescent protein (GFP) and yellow fluorescent protein (YFP) into the seminiferous tubules and in vivo electroporation. We obtained fluorescent spermatozoa only when using the gene of the YFP protein fused to a mitochondrial localization signal peptide. Intracytoplasmic injection into oocytes of these spermatozoa gave fluorescent fetuses and pups. Almost all of the individuals produced from fluorescent spermatozoa were transgenic. We confirmed integration of the gene into chromosomes and its transmission into offspring. This is the first report of gene transfer into germ cells and subsequent production of transgenic offspring.

【 授权许可】

Unknown