Mitochondria from ejaculated human spermatozoa do not synthesize proteins

FEBS Letters

Montoya, Julio¹  Pérez-Martos, Acisclo¹  López-Pérez, Manuel J¹  Ruiz-Pesini, Eduardo¹  Dı́ez-Sánchez, Carmen¹  Enrı́quez, José Antonio¹ 

[1] Departamento de Bioquı́mica y Biologı́a Molecular y Celular, Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain

关键词: Mitochondrial DNA;    Spermatozoon;    Spermatogenesis;    Protein synthesis;    Mitochondrion;    CAP;    chloramphenicol;    DMEM;    Dulbecco's modified Eagle's medium;    EME;    emetine;    OXPHOS;    oxidative phosphorylation;    SDS–PAGE;    sodium dodecyl sulfate–polyacrylamide gel electrophoresis;   

DOI  :  10.1016/S0014-5793(03)01013-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Sperm motility is dependent on mitochondrial ATP production that relies on the coordinated expression of the mitochondrial and nuclear genomes. It is generally accepted that mammalian ejaculated spermatozoa retain the ability to synthesize mtDNA-encoded proteins but not most of the nuclear ones. This implies an asynchronous regulation of the oxidative phosphorylation-related genes encoded by each genome. Trying to investigate this issue, we unexpectedly found that ejaculated human spermatozoa do not synthesize mtDNA-encoded proteins. Moreover, we estimated that the discrepancy between our observations and those published elsewhere was due to a chloramphenicol-sensitive protein synthesis attributed to mitochondria that instead corresponds to contaminating bacteria.

【 授权许可】

Unknown