Induction of apoptosis through oxidative stress-related pathways in MCF-7, human breast cancer cells, by ethyl acetate extract of Dillenia suffruticosa

BMC Complementary and Alternative Medicine

Maznah Ismail¹  Norsharina Ismail¹  Mustapha Umar Imam¹  Rasedee Abdullah²  Yoke Kqueen Cheah³  Nurdin Armania¹  Jhi Biau Foo¹  Latifah Saiful Yazan³  Yin Sim Tor¹ 

[1] Laboratory of Molecular Biomedicine, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia;Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti, Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia;Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra, Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

关键词: Oxidative stress pathway;    Apoptosis;    Cytotoxic;    Breast cancer;    Dillenia suffruticosa;   

Others  :  1220266
DOI  :  10.1186/1472-6882-14-55

 received in 2013-07-15, accepted in 2014-02-10,  发布年份 2014

【 摘 要 】

Background

Breast cancer is one of the most dreading types of cancer among women. Herbal medicine has becoming a potential source of treatment for breast cancer. Herbal plant Dillenia suffruticosa (Griff) Martelli under the family Dilleniaceae has been traditionally used to treat cancerous growth. In this study, the anticancer effect of ethyl acetate extract of D. suffruticosa (EADs) was examined on human breast adenocarcinoma cell line MCF-7 and the molecular pathway involved was elucidated.

Methods

EADs was obtained from the root of D. suffruticosa by using sequential solvent extraction. Cytotoxicity was determined by using MTT assay, mode of cell death by cell cycle analysis and apoptosis induction by Annexin-FITC/PI assay. Morphology changes in cells were observed under inverted light microscope. Involvement of selected genes in the oxidative stress-mediated signaling pathway was explored using multiplex gene expression analysis.

Results

The treatment of EADs caused cytotoxicity to MCF-7 cells in a dose- and time-dependent manner at 24, 48 and 72 hours with IC₅₀ of 76 ± 2.3, 58 ± 0.7 and 39 ± 3.6 μg/mL, respectively. The IC₅₀ of tamoxifen-treated MCF-7 cells was 8 ± 0.5 μg/mL. Induction of apoptosis by EADs was dose- and time- dependent. EADs induced non-phase specific cell cycle arrest at different concentration and time point. The multiplex mRNA expression study indicated that EADs-induced apoptosis was accompanied by upregulation of the expression of SOD1, SOD2, NF-κB, p53, p38 MAPK, and catalase, but downregulation of Akt1.

Conclusion

It is suggested that EADs induced apoptosis in MCF-7 cells by modulating numerous genes which are involved in oxidative stress pathway. Therefore, EADs has the potential to act as an effective intervention against breast cancer cells.

【 授权许可】

   
2014 Tor et al.; licensee BioMed Central Ltd.

【 图 表 】

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