Induction of apoptosis through oxidative stress-related pathways in MCF-7, human breast cancer cells, by ethyl acetate extract of Dillenia suffruticosa

BMC Complementary and Alternative Medicine

Research Article

Yoke Kqueen Cheah¹  Rasedee Abdullah²  Jhi Biau Foo³  Norsharina Ismail³  Yin Sim Tor³  Nurdin Armania³  Maznah Ismail³  Mustapha Umar Imam³  Latifah Saiful Yazan⁴ 

[1] Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra, 43400 UPM, MalaysiaSerdang, Selangor, Malaysia;Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti, Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia;Laboratory of Molecular Biomedicine, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia;Laboratory of Molecular Biomedicine, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia;Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra, 43400 UPM, MalaysiaSerdang, Selangor, Malaysia;

关键词: Dillenia suffruticosa;    Breast cancer;    Cytotoxic;    Apoptosis;    Oxidative stress pathway;   

DOI  :  10.1186/1472-6882-14-55

 received in 2013-07-15, accepted in 2014-02-10,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundBreast cancer is one of the most dreading types of cancer among women. Herbal medicine has becoming a potential source of treatment for breast cancer. Herbal plant Dillenia suffruticosa (Griff) Martelli under the family Dilleniaceae has been traditionally used to treat cancerous growth. In this study, the anticancer effect of ethyl acetate extract of D. suffruticosa (EADs) was examined on human breast adenocarcinoma cell line MCF-7 and the molecular pathway involved was elucidated.MethodsEADs was obtained from the root of D. suffruticosa by using sequential solvent extraction. Cytotoxicity was determined by using MTT assay, mode of cell death by cell cycle analysis and apoptosis induction by Annexin-FITC/PI assay. Morphology changes in cells were observed under inverted light microscope. Involvement of selected genes in the oxidative stress-mediated signaling pathway was explored using multiplex gene expression analysis.ResultsThe treatment of EADs caused cytotoxicity to MCF-7 cells in a dose- and time-dependent manner at 24, 48 and 72 hours with IC50 of 76 ± 2.3, 58 ± 0.7 and 39 ± 3.6 μg/mL, respectively. The IC50 of tamoxifen-treated MCF-7 cells was 8 ± 0.5 μg/mL. Induction of apoptosis by EADs was dose- and time- dependent. EADs induced non-phase specific cell cycle arrest at different concentration and time point. The multiplex mRNA expression study indicated that EADs-induced apoptosis was accompanied by upregulation of the expression of SOD1, SOD2, NF-κB, p53, p38 MAPK, and catalase, but downregulation of Akt1.ConclusionIt is suggested that EADs induced apoptosis in MCF-7 cells by modulating numerous genes which are involved in oxidative stress pathway. Therefore, EADs has the potential to act as an effective intervention against breast cancer cells.

【 授权许可】

Unknown   
© Tor et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.