Study of Cellulase Activity Produced by Penicillium sp., Aspergillus niger and Trichoderma viride on Imperata cylindrica (L.) Beauv. Enrichment Media

MarSave International Symposium 2018: "Strengthening Marine Resilience for Sustainable Development Goals"

生态环境科学

Agustina, D.^1 ; Surtiningsih, T.^1 ; Manuhara, Y.S.W.^1 ; Arifiyanto, A.^1 ; Malewa, M.^2

Department of Biology, Faculty of Sciences and Technology, Airlangga University, Surabaya

60115, Indonesia^1

Department of Law, Faculty of Law, Atmajaya University, Makassar

90224, Indonesia^2

关键词: Aspergillus niger;    Cellulase activity;    Endoglucanases;    Incubation periods;    Lignin structure;    Penicillium sp;    Reducing sugars;    Trichoderma viride;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/253/1/012016/pdf
DOI  :  10.1088/1755-1315/253/1/012016

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

The aim of this study was to determine the value of cellulase activity produced by mould toward filter paperase (FP-ase) and endoglucanase (EG). Penicillium sp., Aspergillus niger and Trichoderma viride utilized as mould isolate. Cogon grass substrates pre-treated with 2% NaOH. It determined the reducing sugar. The respective cellulase activity of each isolate was measured using filter paperase (FP-ase) and endoglucanase (EG). Pre-treatment successfully damaged lignin structure. The most favourable incubation period produced in 7th days. Moreover, it increased surface area of cellulose with a cellulose level of 48.32%, hemicellulose level of 9.88% and lignin level of 1.14%. Aspergillus niger exhibit the highest cellulase activity with enzymes value of 3.97 U/ml for filter paperase (FP-ase) and 4.53 U/ml for endoglucanase (EG). Penicillium sp. reached 3.71 U/ml for filter paperase (FP-ase) and 4.11 U/ml for endoglucanase (EG) while Trichoderma viride got 3.37 U/ml for filter paperase (FP-ase) and 3.38 U/ml for endoglucanase (EG).