Immobilization of Cellulase from Bacillus subtilis UniMAP-KB01 on Multi-walled Carbon Nanotubes for Biofuel Production

Malaysian Technical Universities Conference on Engineering and Technology 2017

Naresh, Sandrasekaran^1 ; Shuit, Siew Hoong^1 ; Kunasundari, Balakrishnan^1 ; Peng, Yong Hoo^1 ; Qi, Hwa Ng^1 ; Teoh, Yi Peng^1

Department of Chemical Engineering Technology, Faculty of Engineering Technology, Universiti Malaysia Perlis (UniMAP), P.O Box 77, D/A Pejabat Pos Besar Kangar, Perlis

01000, Malaysia^1

关键词: Biofuel production;    Carboxymethylcellulose;    Cellulase activity;    Filter paper assays;    Gram-positive bacterium;    Morphological identifications;    Root mean square deviations;    Thermostable cellulase;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/318/1/012008/pdf
DOI  :  10.1088/1757-899X/318/1/012008

来源: IOP

【 摘 要 】

Bacillus subtilis UniMAP-KB01, a cellulase producer was isolated from Malaysian mangrove soil. Through morphological identification it was observed that the B. subtilis appears to be in rod shaped and identified as a gram positive bacterium. Growth profile of isolated B. subtilis was established by measuring optical density (OD) at 600 nm for every 1 hour intervals. Polymath software was employed to plot the growth profile and the non-linear plot established gave the precision value of linear regression, R2of 0.9602, root mean square deviation (RMSD) of 0.0176 and variance of 0.0025. The hydrolysis capacity testing revealed the cellulolytic index of 2.83 ± 0.46 after stained with Gram's Iodine. The harvested crude enzyme after 24 hours incubation in carboxymethylcellulose (CMC) broth at 45°C and 100 RPM, was tested for enzyme activity. Through Filter Paper Assay (FPA), the cellulase activity was calculated to be 0.05 U/mL. The hydrolysis capacity testing and FPA shown an acceptable value for thermophilic bacterial enzyme activity. Thus, this isolated strain reasoned to be potential for producing thermostable cellulase which will be immobilized onto multi-walled carbon nanotubes and the cellulolytic activity will be characterized for biofuel production.