A Consortium of Three Enzymes: Xylanase, Arabinofuranosidase, and Cellulase from Aspergillus sp. which liquefied Coffee Pulp Wastes

9th Annual Basic Science International Conference 2019

自然科学（总论）

Muzakhar, Kahar^1

Biology Dept., Faculty of Mathematics and Natural Sciences, Jember University, Jl. Kalimantan 37, Jember

68121, Indonesia^1

关键词: Active fraction;    Arabinofuranosidase;    Aspergillus sp;    Cellulase activity;    Crude enzymes;    DEAE-Toyopearl 650M;    Solid-state fermentation;    Xylanase activity;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/546/2/022013/pdf
DOI  :  10.1088/1757-899X/546/2/022013

学科分类：自然科学（综合）

来源: IOP

【 摘 要 】

Three enzymes released by Aspergillus sp., identified as xylanase, arabinofuranosidase, and cellulase which hydrolyzed coffee pulp during solid state fermentation (SSF) had been investigated. The active fraction of xylanase activity was detected when purification of crude enzymes was performed on DEAE Cellulofine, while cellulase and arabinofuranosidase were fractionated from the previous active fraction using the DEAE Toyopearl 650M and DEAE Mono-Q columns. All enzymes are optimum active at 55°C and stable at temperatures below 50°C and a range of pH 3-6.5. Optimum cellulase activity at pH 5.5 while xylanase and arabinofuranosidase at pH 5. A consortium of these three enzymes can easily hydrolyze the coffee pulp into simple sugar with a degree hydrolysis 79%.