Green Purification Process of Bacillus Subtilis (LC-9) Multifunction Cellulase

2018 5th International Conference on Advanced Composite Materials and Manufacturing Engineering

Sui, Ming^1,2 ; Li, Chunming^2 ; Li, Guoying^1,3 ; Zhou, Rongqing^1,3

Key Laboratory of Leather Chemistry and Engineering, Ministry of Education and College of Light Industry, Textile and Food Engineering, Sichuan University, Chengdu

610065, China^1

Department of Wine and Food Engineering, Si Chuan Technology and Business College, Du jiang-yan, Sichuan

611800, China^2

National Engineering Laboratory for Clean Technology of Leather Manufacture, Sichuan University, Chengdu

610065, China^3

关键词: Bacillus Subtilis;    Endoglucanases;    Gel electrophoresis;    Micro-crystalline cellulose;    Multiple-group;    Purification process;    Sodium carboxymethyl cellulose;    Various functions;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/394/2/022046/pdf
DOI  :  10.1088/1757-899X/394/2/022046

来源: IOP

【 摘 要 】

The thorough degradation of Cellulose requires the interaction of various functions of cellulase. The single function of cellulase degrades cellulose, which is not only inefficient but also costly. In this paper, the fermentation of Bacillus subtilis LC-9, a cellulase-producing strain, was screened by the laboratory for hydrolysis experiments and found that it could degrade many substrates such as sodium carboxymethyl cellulose, xylan, filter paper, microcrystalline cellulose and pNPG The activity of renatured SDS-PAGE gel electrophoresis showed that the 18.4KDa, 20KDa, 35KDa protein bands at the same time with endoglucanase and xylan; enzyme activity, and thus determine the strain may have produced Multiple groups of multifunctional cellulase. Therefore, the development and research of multifunctional cellulase is of great significance for the degradation and utilization of cellulose.